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Data for The Association of Defensin HNP-2 with Negatively Charged Membranes: A Combined Fluorescence and Linear Dichroism Study Open Access

Abstract from article: The association of defensin HNP-2 with negatively charged membranes has been studied using a new approach that combines fluorescence and linear dichroism (LD) spectroscopies with simulated LD spectra in order to characterise the binding kinetics and bound configurations of the peptide. Binding to membranes composed of mixtures of diacylglycerophosphocholines (PC) with either diacylglycerophosphoglycerol (PG) or diacylglycerophosphoserine (PS) was conducted at lipid:peptide ratios that yielded binding, but not membrane fusion. HNP-2 association with membranes under these conditions was a 2 stage-process, with both stages exhibiting first order kinetics. The fast initial step, with a half-life of < 1 min, was followed by a slower step with a half-life of > 3 min. Conversion between the states was estimated to have an enthalpy of activation of approximately 10 kJ/mol and an entropy of activation of − 0.2 kJ/K/mol. LD spectra corresponding to each of the membrane bound states were generated by non-linear regression using a standard kinetics model. These spectra are interpreted in comparison with spectra calculated using the program Dichrocalc and reveal that the peptide associates with membranes in a small number of stable configurations. All of these configurations have a significant proportion of β-sheet structure residing in the plane of the membrane. Two configurations support structures previously proposed for defensins in membranes.

Descriptions

Resource type

Dataset

Contributors

Creator: Sanderson, John ¹
Creator: Pridmore, Catherine ¹
Creator: Rodger, Alison ²
Contact person: Sanderson, John ¹

¹ Durham University, UK
² University of Warwick, UK

Funder

Engineering and Physical Sciences Research Council

Research methods

Fluorescence spectroscopy; Linear Dichroism spectroscopy; calculations (Dichrocalc, APBS); Förster resonance energy transfer; dynamic light scattering; differential scanning calorimetry; circular dichroism spectroscopy.

Other description

Data generated during a study of defensin HNP-2 association with lipid membranes. Data are grouped by experiment. Information on files is located in each experiment directory, and in some cases where appropriate, in individual directories and files. Data types include: fluorescence (Figs. 2, 4, 6, S1); Linear Dichroism (Fig. 5, S8); Dichrocalc (Fig. 10, 11); APBS (Fig. 12, S17); FRET (Fig. S2, S3); DLS (Fig. S4, S5); DSC (Fig. S6); Circular Dichroism (Fig. S7).

Keyword

peptide
defensin
membrane
lipid

Subject

Location

Language

Cited in

doi:10.1016/j.bbamem.2016.01.014

Identifier

doi:10.15128/z603qx40z
ark:/32150/z603qx40z

Rights

Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA)

Publisher

Durham University

Date Created

2016-01-07

File Details

Depositor

J.M. Sanderson

Date Uploaded

26 January 2016, 10:01:10

Date Modified

17 May 2016, 14:05:35

Audit Status

Audits have not yet been run on this file.

Characterization

File format: zip (ZIP Format)

Mime type: application/zip

File size: 27265903

Last modified: 2016:01:26 10:17:21+00:00

Filename: defensin_data.zip

Original checksum: ce441eeccdbe720d7c7532450ce6ad0c