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Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] Open Access

Hydraulic fracturing plays a major role in the formation of biological cavities during embryonic development, when the accumulation of pressurized fluid leads to the opening of microlumens that fracture cell-cell contacts and later evolve to form a single large lumen. However, the fundamental physical mechanisms behind these processes remain masked by the complexity and specificity of biological systems. Here, we show that adhered lipid vesicles subjected to osmotic stress form hydraulic microlumens similar to those in cells. Combining vesicle experiments with theoretical modelling and numerical simulations, we provide a physical framework for the hydraulic reconfiguration of cell-cell adhesions. We map the conditions for microlumen formation from a pristine adhesion, the emerging dynamical patterns and their subsequent maturation. We demonstrate control of the fracturing process depending on the applied pressure gradients, and the type and density of membrane bonds. Our experiments further reveal an unexpected, passive transition of microlumens to closed buds that suggests a physical route to adhesion remodeling by endocytosis.

Descriptions

Resource type
Dataset
Contributors
Data collector: Torres-Sánchez, Alejandro 1
Creator: Staykova, Margarita 2
Contact person: Staykova, Margarita 2
Data curator: Staykova, Margarita 2
Editor: Staykova, Margarita 2
Data collector: Dinet, Celine 3
Data curator: Dinet, Celine 3
Data collector: Lanfranco, Roberta 4
Data collector: Di Michele, Lorenzo 5
Data collector: Arroyo, Marino 1
1 Universitat Politecnica de Catalunya-BarcelonaTech, 08034 Barcelona, Spain
2 Durham University, UK
3 Laboratoire de Chimie Bacterienne, Institut de Microbiologie de la Mediterranee, CNRS-Aix-Marseille University
4 Department of Physics, Cavendish Laboratory, University of Cambridge
5 Department of Chemical Engineering and Biotechnology, University of Cambridge, UK
Funder
Engineering and Physical Sciences Research Council
European Research Council
Royal Society University Research Fellowship
Wiener-Anspach Foundation
European Commission
Research methods
Other description
Fixed corrupt data file, 2023-12-07.
Keyword
Lipid membranes
Adhesion
Hydraulic fracture
Blisters
Lumen
Coarsening
Subject
Biophysics
Location
Language
Cited in
Identifier
ark:/32150/r2000000048
doi:10.15128/r2000000048
Rights
Creative Commons Attribution 4.0 International (CC BY)

Publisher
Durham University
Date Created

File Details

Depositor
M.M. Staykova
Date Uploaded
Date Modified
7 December 2023, 12:12:01
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Characterization
File format: zip (ZIP Format)
Mime type: application/zip
File size: 21456632908
Last modified: 2023:12:07 12:43:17+00:00
Filename: ResearchData.zip
Original checksum: 7752a9ef39a12ea1131f7be1b4fe4cd6
Activity of users you follow
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User N. Syrotiuk has updated Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] 3 months ago
User N. Syrotiuk has added a new version of Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] 3 months ago
User N. Syrotiuk has updated Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] 6 months ago
User N. Syrotiuk has updated Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] 6 months ago
User N. Syrotiuk has updated Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] 6 months ago
User N. Syrotiuk has updated Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] 6 months ago