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Patterning and dynamics of membrane adhesion under hydraulic stress [dataset] Open Access

Hydraulic fracturing plays a major role in the formation of biological cavities during embryonic development, when the accumulation of pressurized fluid leads to the opening of microlumens that fracture cell-cell contacts and later evolve to form a single large lumen. However, the fundamental physical mechanisms behind these processes remain masked by the complexity and specificity of biological systems. Here, we show that adhered lipid vesicles subjected to osmotic stress form hydraulic microlumens similar to those in cells. Combining vesicle experiments with theoretical modelling and numerical simulations, we provide a physical framework for the hydraulic reconfiguration of cell-cell adhesions. We map the conditions for microlumen formation from a pristine adhesion, the emerging dynamical patterns and their subsequent maturation. We demonstrate control of the fracturing process depending on the applied pressure gradients, and the type and density of membrane bonds. Our experiments further reveal an unexpected, passive transition of microlumens to closed buds that suggests a physical route to adhesion remodeling by endocytosis.

Descriptions

Resource type

Dataset

Contributors

Data collector: Torres-Sánchez, Alejandro ¹
Creator: Staykova, Margarita ²
Contact person: Staykova, Margarita ²
Data curator: Staykova, Margarita ²
Editor: Staykova, Margarita ²
Data collector: Dinet, Celine ³
Data curator: Dinet, Celine ³
Data collector: Lanfranco, Roberta ⁴
Data collector: Di Michele, Lorenzo ⁵
Data collector: Arroyo, Marino ¹

¹ Universitat Politecnica de Catalunya-BarcelonaTech, 08034 Barcelona, Spain
² Durham University, UK
³ Laboratoire de Chimie Bacterienne, Institut de Microbiologie de la Mediterranee, CNRS-Aix-Marseille University
⁴ Department of Physics, Cavendish Laboratory, University of Cambridge
⁵ Department of Chemical Engineering and Biotechnology, University of Cambridge, UK

Funder

Engineering and Physical Sciences Research Council
European Research Council
Royal Society University Research Fellowship
Wiener-Anspach Foundation
European Commission

Research methods

Other description

Fixed corrupt data file, 2023-12-07.

Keyword

Lipid membranes
Adhesion
Hydraulic fracture
Blisters
Lumen
Coarsening

Subject

Biophysics

Location

Language

Cited in

Identifier

ark:/32150/r2000000048
doi:10.15128/r2000000048

Rights

Creative Commons Attribution 4.0 International (CC BY)

Publisher

Durham University

Date Created

File Details

Depositor

M.M. Staykova

Date Uploaded

5 September 2023, 12:09:20

Date Modified

7 December 2023, 12:12:01

Audit Status

Audits have not yet been run on this file.

Characterization

File format: zip (ZIP Format)

Mime type: application/zip

File size: 21456632908

Last modified: 2023:12:07 12:43:17+00:00

Filename: ResearchData.zip

Original checksum: 7752a9ef39a12ea1131f7be1b4fe4cd6