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Lipid bilayer fracture under uniaxial stretch [dataset] Open Access

Most studies on pore formation in lipid membranes focus on lipid vesicles under isotropic tension. These models however fail to replicate the anisotropic stresses encountered by living cells and the complex rheological properties of the cell membrane arising from its interactions with the underlying cytoskeleton. Here, we employ a custom-built device to impose uniaxial stretch on PDMS-supported lipid membranes. We show that in contrast to the circular pores in vesicles, supported membranes under uniaxial loading open elliptical pores that are aligned perpendicularly to the direction of stretch. We discuss the constraints on tension diffusion in supported membranes, and how tension distribution determines the density and the shape of the membrane pores in relation to the applied strain rate and strain magnitude. Our paper shows for the first time that lipid membranes can exhibit a fracture behavior similar to the fracture of soft gels under tensile loading.

Descriptions

Resource type

Dataset

Contributors

Data collector: Goodband, Rachel Joanne ¹
Data curator: Goodband, Rachel Joanne ¹
Editor: Goodband, Rachel Joanne ¹
Creator: Staykova, Margarita ²
Contact person: Staykova, Margarita ²
Data collector: Staykova, Margarita ²
Data curator: Staykova, Margarita ²
Editor: Staykova, Margarita ²

¹ Trinity College Dublin, Ireland
² Durham University

Funder

Engineering and Physical Sciences Research Council

Research methods

Experiments: preparing supporting lipid bilayers and designing a stretching device
Imaging: using confocal and fluorescent microscopes to record the response of the bilayerto stretch
Image analysis: Fiji and Python to analyse and fit the data

Other description

Keyword

lipid bilayers
uniaxial stretch
eliptical pores
elastic fracture

Subject

biophysics
soft matter

Location

Language

English

Cited in

doi:10.1039/d4sm01410c

Identifier

ark:/32150/r1wm117p070
doi:10.15128/r1wm117p070

Rights

Creative Commons Attribution 4.0 International (CC BY)

Publisher

Durham University

Date Created

2024-11-27

File Details

Depositor

M.M. Staykova

Date Uploaded

27 November 2024, 08:11:21

Date Modified

31 January 2025, 09:01:06

Audit Status

Audits have not yet been run on this file.

Characterization

File format: zip (ZIP Format)

Mime type: application/zip

File size: 13462935281

Last modified: 2025:01:30 19:07:43+00:00

Filename: Data repository.zip

Original checksum: ba4afd3765e0823f8a8ef74f0d72e49e