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The Influence of Cholesterol on Melittin Lipidation in Neutral Membranes [dataset] Open Access

The effects of cholesterol on the process of intrinsic lipidation, whereby an acyl chain is transferred from a lipid as donor to a membrane-associated acceptor molecule, have been explored using melittin as the acceptor. Membranes comprising lipids with saturated acyl chains (1,2-dipalmitoyl sn-glycero-3-phosphocholine, DPPC; 1,2-dimyristoyl sn-glycero-3-phosphocholine, DMPC) yielded no acyl transfer, whereas membranes composed of lipids with unsaturated acyl chains (1,2-dioleoyl sn-glycero-3-phosphocholine, DOPC; 1-palmitoyl-2-oleoyl sn-glycero-3-phosphocholine, POPC) produced detectable lipidation activity. For all lipids, inclusion of cholesterol led to a significant increase in lipidation activity, with the greatest effect observed for 20 mol% cholesterol in POPC. In the case of membranes composed of POPC, the inclusion of cholesterol also produced small changes in the selectivity for transfer from the sn-1 vssn-2 positions of the lipid. Qualitatively, for fluid membranes, the trend in lipidation activity exhibits a positive correlation with the bending modulus of the bilayer and is accounted for in terms of the penetration depth of the peptide. Access of water to reactive intermediates also has the potential to influence lipidation rates.

Descriptions

Resource type

Dataset

Contributors

Creator: Britt, Hannah ¹
Contact person: Sanderson, John ¹
Contact person: Mosely, Jackie ¹

¹ Durham University, UK

Funder

Engineering and Physical Sciences Research Council

Research methods

Other description

Keyword

Melittin
Cholesterol
Lipidation

Subject

Cholesterol

Location

Language

Cited in

10.1039/C8CP06661B

Identifier

ark:/32150/r1r494vk21q

Rights

Creative Commons Attribution-ShareAlike 4.0 International (CC BY-SA)

Publisher

Durham University

Date Created

File Details

Depositor

J.M. Sanderson

Date Uploaded

21 November 2018, 12:11:03

Date Modified

7 June 2019, 14:06:48

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File format: x-bzip2 (bzip2 compressed data, block size = 900k, BZ2, Bzip2)

Mime type: application/x-bzip2

File size: 326500806

Last modified: 2018:11:21 12:28:54+00:00

Filename: MS Data Mel Chol.tbz2

Original checksum: a2cab1b3c489bebf5dc27b590e0e5f00