Mass Spectrometry Analysis of Aquaporin-0

Descriptions

Resource type

Collection

Total Items

14

Size

0 Bytes

Contributors

Data curator: Sanderson, John M ¹
Editor: Sanderson, John M ¹
Creator: Ismail, Vian S ¹
Data collector: Ismail, Vian S ¹
Contact person: Mosely, Jackie A ¹
Editor: Mosely, Jackie A ¹
Editor: Quinlan, Roy A ¹

¹ Durham University, UK

Funder

Ministry of Higher Education and Scientific Research, Kurdistan
Fight for Sight UK
Leverhulme Trust

Research methods

Mass spectrometry

Other description

Data files are numbered (1-, 2- etc) according to the publication in which the data first appear. The next term is the species (Bov for bovine, Hum for human) and the lens section (Cortex or Nucleus). Data are grouped according to the type of experiment: LC-MS, LC-MSMS, or LC-MSE (for trypsin digests) and Whole_Protein. Data are presented in original (raw) format, and a more portable format (CDF or mzXML). Those prefixed with '1' are from 10.1016/j.bbamem.2016.06.026 e.g. 1-Bov-Cortex_LC-MSMS_CDF.tbz2 is an archive containing data in CDF format from 10.1016/j.bbamem.2016.06.026 for the bovine lens cortex, analysed by LC-MSMS. Portable formats: CDF files for Synapt G2-S data were generated using the Waters DataBridge program (as msconvert gave mass errors). DataBridge splits each raw data file into 4 CDF files. The LC-MS data are usually in the file suffixed with "01"' LC-MS/MS and LC-MSE data are usually in the file suffixed with "02". mzXML files generated using msconvert contain only the LC-MS portion of the data and may be truncated at longer retention times (where there are no signals of interest). Bovine/Cortex LC-MSE data: contain within the filename the trap collision energy ramp (e.g. .....25_50_CE01.CDF for 25-50 eV). LC-MSMS data: as LC-MSE data, plus the filename contains the mass of the parent ion (e.g. ...MSMS998....CE01.CDF for fragmentation of m/z 972) LC-MS data: e.g. VSI_AQPO0C1_TRYP_DIG_DTT_SOD_AZID_C18_111214 was an outer cortex sample (C1) digested with trypsin, with a buffer containing DTT and sodium azide, run on a C18 column on 11th Dec 2014; VSI_TRYPDIGEST_AQPO1_DTT_SODAZIDE_C18_220714 was an outer cortex sample (01) digested with trypsin, with a buffer containing DTT and sodium azide, run on a C18 column on 22nd July 2014. Whole (intact) Protein data: e.g. VSI_AQPO0C1_C4_SOD_AZ_PFOA_191214 was an outer cortex (01) sample, buffer containing sodium azide and PFOA, run on a C4 column on 19th Dec 2014. Bovine/Nucleus LC-MS data: e.g. VSI_AQPO0N_TRYP_DIG_DTT_SODAZID_C18_NEW_121214 was an nuclear sample (N) digested with trypsin, with a buffer containing DTT and sodium azide, run on a C18 column on 12th Dec 2014. Human/Cortex LC-MSE data: contain within the filename the sample injection volume for the run (e.g. ...4UL....CE01.CDF for 4 microlitres). LC-MSE data: contain within the filename the age and gender of the donor (e.g. ...M22....CE01.CDF for 22 year old male). LC-MSE data: contain within the filename the trap collision energy ramp (e.g. .....30_50_CE01.CDF for 30-50 eV). LC-MSMS data: as LC-MSE data, plus the filename contains the mass of the parent ion (e.g. ...MSMS972....CE01.CDF for fragmentation of m/z 972) HeliQuest Data Output files for h-AQP0 are in the directory Human/HeliQuest.

Keyword

lipidation
lipid
mass spectrometry
lens
posttranslational modification
aquaporin
membrane protein

Subject

Lipids
Post-translational modification
Membranes (Biology)
Membrane proteins

Language

English

Cited in

doi:10.1016/j.bbamem.2016.06.026

Identifier

ark:/32150/r12j62s4846
doi:10.1016/j.bbamem.2016.06.026

Publisher

Durham University

Date Created

2016-07-11