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A minimalist model lipid system mimicking the biophysical properties of Escherichia coli’s membrane [dataset] Open Access

Biological membrane are highly complex systems that are of fundamental importance to the development and survival of organisms. Native membranes typically comprise different types of lipids, biomolecules and proteins assembled around a lipid bilayer structure. This complexity can render investigations challenging, with many studies relying on model membranes such as artificial vesicles and supported lipid bilayers (SLBs). The purpose of a model system is to capture the desired dominant features of the native context while remaining uniquely defined and simpler. Here, we search for a minimal lipid-only model system of the Escherichia coli inner membrane. We aim to retain the main lipidomic components in their native ratio while mimicking the membrane thermal and mechanical properties. We design a collection of candidate model systems reflecting the main aspects of the known native lipidomic composition and narrow down our selection based on the systems’ phase transition temperature. We further test our candidate model systems by independently measuring their elastic properties. We identify 3 ternary model systems able to form stable bilayers that closely mimic E. coli’s inner membrane lipid composition and mechanical properties. These model systems are made of commercially available synthetic 16:0-18:1 phosphatidylethanolamine (POPE), 16:0-18:1 phosphatidylglycerol (POPG), and 16:0-18:1 Cardiolipin (CL). We anticipate our results to be of interest for future studies making use of E. coli models, for example investigating membrane proteins’ function or macromolecule-membrane interactions.

Descriptions

Resource type

Dataset

Contributors

Creator: Tormena, Nicolo ¹
Data collector: Tormena, Nicolo ¹
Creator: Voitchovsky, Kislon ¹
Data curator: Voitchovsky, Kislon ¹
Pilizota, Teuta ²

¹ Durham University, UK
² Unversity of Edinburgh, UK

Funder

Engineering and Physical Sciences Research Council

Research methods

Differential Scanning calorimetry Optical microscopy Atomic Force microscopy

Other description

Keyword

Subject

Biophysics

Location

Language

Cited in

doi:10.1101/2024.09.29.615671

Identifier

ark:/32150/r22r36tx61b
doi:10.15128/r22r36tx61b

Rights

Creative Commons Attribution 4.0 International (CC BY)

Publisher

Durham University

Date Created

File Details

Depositor

K.D. Voitchovsky

Date Uploaded

1 October 2024, 16:10:10

Date Modified

2 October 2024, 13:10:54

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Characterization

File format: zip (ZIP Format)

Mime type: application/zip

File size: 185817646

Last modified: 2024:10:02 13:21:13+01:00

Filename: SupportingData.zip

Original checksum: 9cec96045e46236dda018e3541cc6c4c